Ravindra Kumar is a skilled expert, who works in Merck main office of which is in 60. as a Associate Director, Global Technical Operations, Coe since 2018. Ravindra has earned professional experience in more than 11 positions at different companies. Ravindra Kumar went to the Jawaharlal Nehru University and got education from 2000 to 2004. This professional has such professional qualifications as Certificate in effective mentoring. University of North Carolina, Chapel Hill, NC. Durham, North Carolina is the city, where this expert was lastly known to be situated. The person's email address and number can be accessed through this website on demand.
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Ravindra Kumar
Last updated Jun 15, 2024
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Workplace
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Work History
Associate Director, Global Technical Operations, Coe
2000 Galloping Hill Rd, Kenilworth, NJ 07033
from Mar 2018
Lead Scientist Iv, Bio-Cmc
206 Carnegie Ctr Dr, Princeton, NJ
Served as a bioassay and immunoassay SME and provided regulatory and technical expertise.
Lead multiple projects for startup to big biopharmaceutical companies.
Provided technical trainin...
Oct 2015 — Mar 2018
Analytical Technology Scientist I
225 Binney St, Cambridge, MA 02142
Performed bioassays for biologics in GMP compliant laboratory.
Designed statistically sound experiments using DOE and analyzed complex data using JMP.
Wrote, reviewed, and signed off on v...
Apr 2015 — Oct 2015
Bench Scientist
North Carolina
Development of assays for autoimmune and inflammatory diseases of skin.
Developed ex-vivo and in-vitro assays for target engagement studies of pre-clinical assets.
Developed and validated...
2014 — 2015
Research Associate
Chapel Hill, NC
Mentor: Dr. Joseph S. Pagano
Study of Epstein-Barr Virus (EBV) deubiquitinating enzyme and its role in the viral cytolytic cycle.
Purify EBV deubiquitinase and PCNA ubiquitinating enzymes...
Aug 2012 — Feb 2014
Visiting Researcher
Investigated therapeutic potential of anti-TNF-alpha ribozyme in experimentally induced arthritis in mice.
Gained knowledge and expertise in collagen-induced arthritis mouse model and immu...
May 2002 — Oct 2003
Education
1994 — 1997
Occupations
Executive
Deputy Director
Scientist
Deputy Manager
Chief Executive
Skills
Protein Purification
Cell Culture
Western Blotting
Cell
Protein Chemistry
Sds Page
Microscopy
Biochemistry
Elisa
Fluorescence
Immunology
Confocal Microscopy
Cancer
Pcr
Molecular Biology
Qpcr
Dna
Protein Expression
Cell Biology
Cell Based Assays
Life Sciences
Rt Pcr
Facs
Fluorescence Microscopy
Molecular Cloning
Purification
In Vivo
Tissue Culture
Histology
Dls
Fplc
Fret
Immunoprecipitation
Itc
Arthritis Mouse Model
Immunohistochemistry
Immunofluorescence
Animal Models
Gxp
Lims
Method Transfer
Receptor Binding Assays
Bioassay Validation
Trackwise
Oos
Capa
Immunoassays
Pla
Real Time Polymerase Chain Reaction
Proficient in KTA chromatography
protein purification and characterization
spectrophotometric enzyme assays
dynamic light scattering (DLS)
gel electrophoresis
PAGE
IEF
CE
western blot
and ELISA. Development of kinetic assays and bioconjugation protocols for enzymes
protein-protein and protein- DNA interactions detection with ITC
SPR
FRET
cross-linking
and single-molecule FRET. Expertise in basic and advanced molecular biology methods
including
but not limited to
gene cloning
site-directed mutagenesis
real-time PCR
ribonuclease protection assay
in vitro transcription and translation
T7 phage display. Experience with human and murine cell culture
FACS analysis
transfection
stable cell line construction
fluorescence and confocal microscopy. Expertise and in-depth knowledge of arthritis mouse model
administration of drugs via various routes
surgery
organ collection and downstream process such as histology and total RNA purification. Monoclonal antibody isolation
antibody labeling with biotin and fluorescence dyes
immunoprecipitation
and immunofluorescence imaging. Expertise in Unicorn software
Graph Pad Prism
Origin
Ms-Office suite
Photoshop
Adobe Illustrator
Dreamweaver
and Macromedia Flash.